Journal: Pharmaceuticals
Article Title: HPLC-ESI-QTOF-MS/MS-Guided Profiling of Bioactive Compounds in Fresh and Stored Saffron Corms Reveals Potent Anticancer Activity Against Colorectal Cancer
doi: 10.3390/ph19010149
Figure Lengend Snippet: Cytotoxic effects of HEES ( A , C ) and HEEF ( B , D ) on the colon cancer cell lines SW480 ( A , B ) and T84 ( C , D ), respectively, determined using the crystal violet assay at different concentrations of the extract (10, 50, 100, 250 µg/mL). HEEF: hydroethanolic extract from fresh corms. HEES: hydroethanolic extract from stored corms. Data are expressed as mean ± SEM. Statistical significance compared with the control group (0 µg/mL) is indicated as * p < 0.05, ** p < 0.01, and *** p < 0.001.
Article Snippet: Colon cancer cell lines SW480 and T84 were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA), cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, Life Technologies, Madrid, Spain) supplemented per liter with 10% fetal bovine serum (FBS, Gibco, Life Technologies, Spain), 1% penicillin/streptomycin (100 U/mL penicillin and 100 μg/mL streptomycin, Sigma, Madrid, Spain), and 1% L-glutamine (2 mM) (Gibco, Life Technologies, Spain).
Techniques: Crystal Violet Assay, Control